Table 3.

Leading-edge proteins contributing to the strongest negatively and positively enriched mitochondrial pathways in old female HFpEF cardiomyocyte mitochondria. Pre-ranked GSEA of MitoCarta 3.0 pathways was performed comparing old female HFD+L-NAME-treated mice with age-matched RD controls. The four pathways with the most negative normalized enrichment scores (NES) and the three pathways with the most positive NES were selected for presentation. The leading-edge protein subset that contributes most to the enrichment score for each pathway is shown. Negative NES indicates relative suppression in HFpEF, whereas positive NES indicates relative enrichment in HFpEF. FDR-adjusted P values are provided for each pathway.

OXPHOS subunits	OXPHOS	Complex I	Complex I subunits	Lipid metabolism	Detoxification	ROS and glutathione metabolism
NES = −1.846	NES = −1.780	NES = −1.729	NES = −1.722	NES = 1.686	NES = 1.595	NES = 1.511
FDR = 0.018	FDR = 0.018	FDR = 0.027	FDR = 0.032	FDR = 0.018	FDR = 0.039	FDR = 0.083
Cox6a1	Fmc1	Tmem186	Ndufb2	Ephx2	Ephx2	Cat
Cox7a2l	Cox6a1	Ndufaf2	Ndufa7	Acad11	Cat	Mgst1
Cox8b	Higd1a	Ndufaf7	Ndufab1	Hsd17b4	Mgst1	Aifm2
Cox7b	Uqcc1	Tmem126b	Ndufb11	Pnpla8	Aifm2	Sod1
Hccs	Cox7a2l	Ndufb2		Ehhadh	Mtarc2
Uqcr11	Cox17	Foxred1		Scp2	Sod1
Cyct	Tmem186	Ndufaf3		Acot2
Ndufb2	Pnkd	Ecsit		Phyh
mt-Co3	Atpaf2	Tmem70		Echdc1
Cox7c	Cox10	Dmac2		Plscr3
Ndufa4	Cox11	Ndufaf5		Amacr
Ndufa7	Ndufaf2	Ndufa7		Ech1
Cyc1	Ndufaf7	Ndufab1		Acaa1a
Ndufab1	Cox8b	Ndufb11		Acadvl
Uqcr10	Higd2a			Gpam
Ndufb11	Cox7b			Crot
Cox6a2	Hccs			Mlycd
Dmac2l	Uqcr11			Dhrs1
Ndufv2	Coa3			Acsl1
	Cox15			Cpt2
	Uqcc3			Cyp27a1
	Tmem126b			Cpt1b
	Pet100			Eci2