Figure 3. SP-NK1R signaling increases transcripts for proteins of the NLRP3 inflammasome and synthesis of pro–IL-1β and TNF-α in mouse keratinocytes.

(A) Quantification by qRT-PCR of transcripts for proteins of the NLRP3 inflammasome in COCA cells at indicated times following treatment with SarSP (10^–7 M) or vehicle. (B) Detection of pro–IL-1β and TNF-α protein by ELISA in COCA cells at indicated times following treatment with SarSP (10^–7 M) or vehicle. (C) Comparative analysis by qRT-PCR of pro–IL-1β, pro–caspase-1, TNF-α, and IL-6 transcripts in primary cultures of keratinocytes isolated from K14^Cre/+ NK1R^KO (K14-NK1R^KO) or littermate (Litt) control mice. In A and B, each circle represents 1 culture of COCA cells (n = 3 and n = 4, respectively). In C, each circle represents a primary keratinocyte culture from 1 mouse (n = 3). Means ± 1 SD were analyzed by 1-way ANOVA and post hoc Student-Newman-Keuls test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.