Table 2.

Field samples used for validation of the real-time RT-PCR assay.

No.	Region	District	Rural district	Location / Farm	Coordinates	Animal type and age
1	Zhetysu	Koksu	Aynabulak	Aynabulak	44°37′14.0”N, 77°59′37.0″E	5 small ruminants (sheep and goats), 3–4 years
2	Zhetysu	Kerbulak	Shankhanay	Koyankoz, farm “Nurly Tan”	44°14′55.0″N, 78°14′52.4″E	5 cattle, 3–6 years
3	Almaty	Uygur	Taskarasu	Taskarasu	43°46′10.2″N, 79°27′00.8″E	5 cattle, 3–6 years
4	Almaty	Uygur	Chundzha	Chundzha	43°32′19.8″N, 79°28′03.7″E	5 small ruminants (sheep), 2–4 years
5	Turkestan	Sozak	Sholakkorgan	Sholakkorgan	43°45′53.6”N, 69°10′46.5″E	5 small ruminants (sheep), 4–5 years
6	Turkestan	Sozak	Sholakkorgan	Sholakkorgan	43°45′53.6”N, 69°10′46.5″E	4 cattle, 2.5–5 years
7	Turkestan	Saryagash	Kashar	Private household	41°27′49.2”N, 69°10′51.0″E	cattle (bull), 2.5 years
8	Zhambyl	Zhambyl	Karakemer	Karakemer village, private household	43°25′31.2″N, 77°37′19.0″E	small ruminant (sheep), 4 years
9	Zhambyl	Zhambyl	Karasu	Ashybulak village, private household	43°04′55.9”N, 71°12′05.1″E	5 cattle, 4–7 years
10	Zhambyl	Sarysu	Saudakent	Baikadam village, private household	43°44′03.5″N, 69°55′11.6″E	3 small ruminants (sheep), 4–5 years
11	Zhambyl	Sarysu	Zhayylma	Zhayylma village, private household	43°45′32.9″N, 69°47′14.9″E	small ruminant (goat), 4 years
12	Pavlodar	Maysky	Kentubek	Kentubek, farm “Akzhar Ondiris”	51°30′32.0”N, 77°29′12.0″E	10 cattle, 2–5 years
13	North Kazakhstan	Magzhan Zhumabayev	Avangard	Roshchina, farm “Bereke SKO”	54°40′12.1”N, 70°21′38.6″E	10 cattle, 2–6 years