Table 3.

Oligonucleotide primers and probes used for detection of Bluetongue virus.

No.	Primer/Probe	Sequence (5′–3′)	GC content (%)	Product size (bp)
1	BTV10/1-F	YGCRATTCACGGYGTAAA	47.2	112
	BTV10/1-R	CCGTGCAAAGTGGRCTACACR	52.4
	BTV10/1-Probe	ATGCCAGGSCCGCTCACACG	65.0
2	BTV10S/2-F	SCCGCTCACACGTGCKATY	57.9	119
	BTV10S/2-R	ACCGACACACCRCHYRTGTAAC	56.0
	BTV10S/2-Probe	DCGCCGCYTAYGYRTAGYCCACT	60.1
3	BTV-VP7-F	ACRCGWGCDATYGCDCGCG	66.7	121
	BTV-VP7-R	GTAAGTGTAATYTHAGAGACGT	35.6
	BTV-VP7-Probe	CRCTTTGCACGGGTGTGGGTT	59.5
4	BTV-VP7/1-F	GTTAAAAATCTHDAGAGAT	24.6	109
	BTV-VP7/1-R	GCRCATGCYCGCATYACRG	63.2
	BTV-VP7/1-Probe	ACACTATCGCNGCAAGRGCRC	59.5