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Comparative amplification efficiency of different primer–probe sets.
| No. | Primer–probe set | PC1 (Ct, Mean ± SD) | PC2 (Ct, Mean ± SD) | NC |
|---|---|---|---|---|
| 1 | BTV10/1 | 17.44 ± 0.32 | 19.70 ± 0.28 | ND |
| 2 | BTV10S/2 | 40.56 ± 0.45 | 39.98 ± 0.40 | ND |
| 3 | BTV-VP7 | 26.94 ± 0.41 | 27.70 ± 0.35 | ND |
| 4 | BTV-VP7/1 | ND | ND | ND |
Quantitative amplification data obtained using the Cycling A. Green channel. PC1, plasmid DNA; PC2, BTV RNA (Vero cells); NC, negative control; ND, not detected.
