Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Characteristics of the selected BTV10/1 primer-probe set for detection of Bluetongue virus by Real-Time RT-PCR-Blutanque-RIBSP.
| Target gene | Type | Tm (°C) | Length (nt) | GC content (%) | Sequence (5′–3′) | Product size (bp) |
|---|---|---|---|---|---|---|
| VP7 | Forward | 60.3 | 18 | 47.2 | YGCRATTCACGGYGTAAA | 112 |
| VP7 | Reverse | 59.8 | 21 | 52.4 | CCGTGCAAAGTGGRCTACACR | |
| VP7 | Probe | 59.5 | 20 | 65.0 | ATGCCAGGSCCGCTCACACG |
