Table 6.

Analytical sensitivity of the real-time RT-PCR-Blutanque-RIBSP assay.

No.	Dilution	Plasmid DNA concentration	Ct (replicate 1)	Ct (replicate 2)	Ct (replicate 3)	Mean Ct ± SD	CV (%)
1	10−1	3 μg	18.2	18.6	17.8	18.2 ± 0.40	0.45
2	10−2	0.3 μg	20.1	21.1	22.0	21.06 ± 0.95	2.20
3	10−3	3 ng	24.3	23.7	24.1	24.03 ± 0.31	1.29
4	10−4	0.3 ng	27.9	27.4	28.3	27.86 ± 0.45	1.29
5	10−5	3 pg	30.2	35.1	34.5	33.26 ± 2.66	1.61
6	10−6	0.3 pg	40.2	ND	ND	—	—

ND, not detected; pg, picogram; ng, nanogram; μg, microgram.