Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Comparative results of real-time RT-PCR assay and commercial test for detection of Bluetongue virus.
| Category | Ct range | Reference assay (commercial kit) | Developed assay |
|---|---|---|---|
| Positive | 10–35 | 8 | 8 |
| False positive | 35–40 | 1 | 1 |
| False negative | >38 | 3 | 2 |
| Negative | No Ct or >40–45 | 53 | 53 |
| Total | — | 61 | 61 |
