Figure 2.
Low levels of transgene expression in the brain following IT sc-rAAV delivery. (A.) EGFP expression in the brain was undetectable by Western blotting. Rats were injected IT with sc-rAAV vectors of different serotypes as indicated (3 × l09 particles/animal). (B.) Low levels of EGFP expression could be detected in the brain by qPCR (sc-rAAV2/l, 3 × l09 particles/animal). The standard curve demonstrates that the median Tc for EGFP expression in the brain corresponded to only 2 × 10-4-fold the expression found in the lower SC.