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. 2006 Jan 30;7:9. doi: 10.1186/1471-2202-7-9

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Copyright © 2006 Cahill et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Plasmid-based expression of shRNA-SNAP-25. A) Plasmid map of pG418-shRNA, a plasmid designed to express shRNAs from the mouse U6 promoter. B) The two 56-base deoxyoligonucleotides used in the construction of pG418-shRNA-SNAP-25 are shown as they would be paired after annealing. The labels above and below the oligonucleotides indicate the source or the function of the nucleotides in the indicated regions. C) The predicted stem-loop structure of shRNA-SNAP-25 expressed from pG418-shRNA-SNAP-25.