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. 2006 Feb 18;34(4):1084–1091. doi: 10.1093/nar/gkj503

Figure 1.

Figure 1

RecJ binds 5′ single-stranded tails. Mobility shift assays were performed with purified wild-type RecJ protein complexed to 33P-labeled DNA substrates. Complexes were resolved on a native acrylamide gel. Assays were performed with 5 nM of a 65 bp double-stranded substrate (A) or 5′ single-stranded tailed substrates with 4 (B), 10 (C) and 20 (D) unpaired 5′ nucleotides. RecJ concentration varies from 5 to 200 nM.