Figure 4.

RecJ binds monomerically to DNA. Electrophoretic mobility shift assays with 5 nM oligonucleotide A treated with 100 nM RecJ alone, RecJf alone or with a mixture of the two (A). An identical experiment, except using the 10 nt 5′ tailed substrate (B). Two distinct shifts are noted, owing to the ∼40 kDa difference in molecular weight between RecJ and RecJf.