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. 2006 Feb 18;34(4):1084–1091. doi: 10.1093/nar/gkj503

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© The Author 2006. Published by Oxford University Press. All rights reserved

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RecJ degrades DNA to a junction. (A) DNA substrate (1.7 pmol) containing a 5′ single-stranded tail of 20 nt in length and a radiolabel on the 3′ end was degraded with 4 pmol purified native RecJ protein (+RecJ). The products were then resolved on a denaturing acrylamide gel alongside a control with no degradation (−RecJ). Single-stranded size markers of 65, 61, 55 and 45 nt in length were resolved in adjacent lanes. (B) DNA substrate (80 fmol) containing a single-stranded tail of 10 nt and a radiolabel on the 3′ end was degraded with 170 fmol purified native RecJ protein for the indicated times (0–20 min) and resolved as before. Adjacent size markers were 65, 55, 45 and 30 nt.