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. Author manuscript; available in PMC: 2006 Feb 21.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2005 Aug;46(8):2781–2789. doi: 10.1167/iovs.05-0022

Figure 4. Effect of inhibition of eNOS and nNOS on α1-adrenergic agonist-stimulated protein secretion.

Figure 4

A. Lacrimal gland acini were preincubated with the eNOS inhibitor L-NAME or its inactive isomer D-NAME at the indicated concentration for 20 minutes prior to stimulation with the α1-adrenergic agonist phenylephrine (10−4 M) for 20 minutes. Peroxidase secretion was then measured. Values are mean ± SEM from 4–6 independent experiments. * indicates statistically significant difference from basal. # indicates statistically significant difference from phenylephrine alone. B. Acini were preincubated with the nNOS inhibitors thiocitrulline or methyl-thiocitrulline at the indicated concentration for 20 minutes prior to stimulation with the α1-adrenergic agonist phenylephrine (10−4 M) for 20 minutes. Peroxidase secretion was then measured. Values are mean from 2 independent experiments.