Table 1.
Radiation and viral doses needed to kill various fractions (Fa) of A549 and H1299 cells.
Fraction Affected (Fa) | Cell line | Radiation alone (Gy) | NV1066 alone (MOI) | Radiation dose reduction index | NV1066 dose reduction index | Combination Index value |
---|---|---|---|---|---|---|
LD10 | A549 | 3.2 | 0.0003 | 6160 | 11 | 0.0918 |
H1299 | 3.6 | 0.14 | 6.2 | 4.9 | 0.3669 | |
LD20 | A549 | 4.7 | 0.002 | 1161 | 10 | 0.1044 |
H1299 | 4.8 | 0.28 | 4.6 | 5.4 | 0.4021 | |
LD30 | A549 | 6 | 0.007 | 383 | 9 | 0.1150 |
H1299 | 5.8 | 0.45 | 3.8 | 5.8 | 0.4373 | |
LD40 | A549 | 7.5 | 0.02 | 154 | 8 | 0.1266 |
H1299 | 6.8 | 0.66 | 3.2 | 6 | 0.4748 | |
LD50 | A549 | 9 | 0.05 | 67 | 8 | 0.1427 |
H1299 | 7.9 | 0.93 | 2.8 | 6.5 | 0.5170 | |
LD60 | A549 | 10.9 | 0.14 | 29 | 7 | 0.1702 |
H1299 | 9.1 | 1.32 | 2.4 | 6.9 | 0.5679 | |
LD70 | A549 | 13.4 | 0.4 | 12 | 7 | 0.2306 |
H1299 | 10.7 | 1.93 | 2 | 7.3 | 0.6348 | |
LD80 | A549 | 17.3 | 1.4 | 4 | 6 | 0.4165 |
H1299 | 12.9 | 3.07 | 1.6 | 7.8 | 0.7355 | |
LD90 | A549 | 25.3 | 9.8 | 0.7 | 6 | 1.5524 |
H1299 | 17.2 | 6.2 | 1.2 | 8.7 | 0.9360 |
Dose reduction index is the –fold of dose reduction possible to achieve the same cell kill if radiation and virus are used in combination. LDx = Lethal dosex, dose needed to kill x% of cells, MOI = multiplicity of infection.