FIGURE 1.

Experimental design and data analysis workflow. Schematic representation of the experimental workflow for cytotoxicity and genotoxicity assessment. HL60 and HepG2 cells were treated with increasing concentrations of the test chemicals. Based on cytotoxicity results, selected concentrations were used for the comet assay to assess genotoxicity. Each slide contained two spots per experiment, and each sample was measured in duplicate, one positioned at the front and one at the back of the electrophoresis apparatus. For comet analysis, only slides with more than 100 cells per concentration per experiment were included. Adapted from Sherif et al. (2026) with permission (Sherif et al., 2026b).