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. 2002 Sep 1;30(17):3748–3753. doi: 10.1093/nar/gkf486

Figure 3.

Figure 3

Sequence-specific protection of pSPORT I plasmid DNA from restriction endonuclease BssHII hydrolysis by incubation with ImPyImLDu86 (7). (A) A BssHII restriction map of pSPORT I DNA and schematic representation of the binding mode of conjugate 7 at BssHII hydrolysis sites. The Py and Im rings are represented by open and closed circles, respectively; the triangle represents segment A of DU-86. (B) Agarose gel (1.2%) electrophoresis of triamide–CPI conjugate-treated pSPORT I DNA after BssHII (2.5 U) digestion (lanes 3–12). Lane 1, 250-bp DNA marker; lane 2, pSPORT I DNA control; lane 3, no drug treatment; lanes 4–11, 5 µM, 1 µM, 500 nM, 250 nM, 100 nM, 50 nM, 10 nM, 1 nM concentrations of conjugate 7; lane 12, 5 µM conjugate 14. sc, supercoiled DNA; li, linear DNA; oc, open circle DNA.