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Hydrolysis of pQBI PGK by Eco52I in the presence of ImImPy. (A) The chemical structure of ImImPy. (B) Agarose gel (1.2%) electrophoresis of the products of Eco52I hydrolysis of pQBI PGK DNA in the presence of various concentrations of ImImPy (lanes 2–5). Lane 1, supercoiled pQBI PGK control (sc); lane 2, 5 µM ImImPy; lane 3, 2 µM; lane 4, 1 µM; lane 5, 500 nM; lane 6, linear pQBI PGK control (li). oc, open circle DNA.
