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. 2002 Sep 1;30(17):3818–3830. doi: 10.1093/nar/gkf501

Figure 4.

Figure 4

(A) Gel shift competition assays. ZfHpaII probe (1.0 nM) was incubated with M.HpaII or Zf.M.HpaII enzymes (1.7 nM) (see Materials and Methods). Lane C, no competitor DNA; subsequent lanes contain competitor DNA at final concentrations of 15.5, 30.8, 77, 154, 308, 616 and 1078 nM. The competitor DNA used is indicated on the right of each gel; the protein assayed in each case is indicated on the left of each gel. For the self-competition experiment, the full retardation gel is shown. For all subsequent experiments, only that portion of the gel containing the retarded probe is shown. (B) Analysis of triplicate binding data described in (A) above.