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. 2002 Sep 1;30(17):3818–3830. doi: 10.1093/nar/gkf501

Figure 5.

Figure 5

Gel retardation competition analysis of the interaction of Zf.M.HpaII with pre-methylated target site DNA. (A) ZfHpaII probe was incubated with Zf.M.HpaII enzyme as in Figure 3. Lane C, no competitor DNA; subsequent lanes contain competitor DNA at final concentrations of 15.5, 30.8, 77, 154, 308, 616 and 1078 nM. The competitor DNA used is indicated on the right of each gel. (B) Analysis of triplicate binding data described in (A). Oligonucleotides ZM3, ZM5 and ZM5/3 are identical to oligonucelotide ZfHpaII but contain methylcytosine at the target site (i.e. mCCGG) on the top, bottom and both strands, respectively.