Table 1. Oligodeoxynucleotides used during protein–DNA binding and methylation studies.

Oligonucleotide	Sequence (5′→3′) (top strand)
Zf	TTCCATGGAGACGCAGAAGCCCTTCAGTTCCAG
ZfHhaI	GATGGCGAGGGC*GCCTTCCATGGAGACGCAGAAGCCCTTCAGCGGCCA
ZfHpaII (Sep16)	GATGGCGAGGCC*GGCACACATGGAGACGCAGAAGCCCTTCAGCGGCCA
HhaI	GGGATGGCCAGGGCGCCTTCCATGGAGAC
HpaII	GATGGCGAGGCCGGCTTCCATGGAGACGCAGGTGAGTTCCTCACGCCA
HpaII*	GTACGAGCAGCTCCCGGGTCAGTCTGCCTA
Non-Sp	GCTGATTGCGAGGGTACCTTCCATGTAACTATTCTCTAACTTCAGG
ZhpaII	GATGGCGAGGCCGGAAGCCCTTCAGCGGCCA
Sep 2	GATGGCGAGGCCGGCAGAAGCCCTTCAGCGGCC
Sep 5	GATGGCGAGGCCGGACGCAGAAGCCCTTCAGCGGCCA
Sep 6	GATGGCGAGGCCGGGACGCAGAAGCCCTTCAGCGGCCA
Sep 11	GATGGCGAGGCCGGATGGAGACGCAGAAGCCCTTCAGCGGCC
Sep 17	GATGGCGAGGCCGGTGTGCTGTAGCTACGCAGAAGCCCTTCAGCGGCCA
Sep 22	GATGGCGAGGCCGGTGTGCTGTAGCTGGAGGACGCAGAAGCCCTTCAGCGGCCA
Sep 25	GCTCATCCACTGCGCACTCATGTCAGACTACCCGGTGTGCTGTAGCTACTAAGTGACGCAGAAGCCCCTCAGTGC
Sep 33	GCTGCTCATCCATCCCGGCGCTTACTGGTGTAGCTAAGTCTAAGTGACGCAGAAGCCCCTCAGTGC
Sep 39	GCTCATCCACTCCCGGTGTCAGACTAGCGCTGTCTGTAGCTACTAAGTGACGCAGAAGCCCCTCAGTGC
Sep 44	GCTCATCCACTCCCGGACTCATGTCAGACTAGCGCTGTGCTGTAGCTACTAAGTGACGCAGAAGCCCCTCAGTGC

Both the C5-Mtase and zinc finger recognition sites are underlined. ZfHhaI and ZfHpaII were also synthesised with 5-methyl-2′-deoxycytosine at the target cytosine in the C5-Mtase recognition site. The target base is asterisked in the respective sequences. Where indicated in the text, 5-methyl-2′-deoxycytosine was also present in the bottom strand at the corresponding symmetrically opposite target cytosine. [Only the top (5′→3′) strands are shown.] Base separation between subsites is measured from (and includes) the target cytosine in the HpaII site up to the first base before the zinc finger site.