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(A) Schematic presentation of the primer extension, restriction digestion and heat denaturation procedures and corresponding products. (B) TBE–agarose gel (0.6%) analysis of the primer extension product containing statistically incorporated NH2-dT:dT using 7249 nt M13 plasmid as template and the corresponding MScI restriction digestion product. (C) Heat-denatured samples of (B) analyzed by 0.6% TBE–agarose gel electrophoresis.
