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. 2002 Sep 1;30(17):3839–3847. doi: 10.1093/nar/gkf504

Figure 2.

Figure 2

Endonuclease assay of the products of the iceA1 genes in CH4 and 60190. (A) Assay of crude extracts from E.coli strain ER2169/pSYX20-NlaIIIM (1) and ER2169 carrying pQX60A (2), pQX60T (3) and pQXCH4 (4). Crude extracts (0.1–3 µl) were used to digest 1 µg bacteriophage λ DNA and the digested DNA was electrophoresed on a 1% agarose gel. Arrows indicate the position of undigested λ DNA in the gels. (B) Assay of the purified CH4 iceA1 endonuclease. Parallel digestion of pUC19, pBR322 and φX174 DNA was performed with both the NlaIII and the CH4 iceA1 endonucleases. The DNA standards are labled L.