| Escherichia coli |
|
|
| ER2169 |
mcrBC, λDE3, pLysS, pSYX20-NlaIIIM, CmR, KanR
|
(15) |
| Helicobacter pylori |
|
|
| 60190 |
iceA1, cagA+, vacA s1a m1 |
(9,10,12) |
| CH4 |
iceA1, cagA+, vacA s1a m2 |
(9,10,12) |
| Plasmids |
|
|
| pSYX22 |
pET11-derived vector, AmpR
|
(15) |
| pQXCH4 |
CH4 iceA1 in pSYX22 |
This study |
| pQX60A |
60190 iceA1 starting from ATG site, in pSYX22 |
This study |
| pQX60T |
60190 iceA1 starting from TTG site, in pSYX22 |
This study |
| pQX60R |
The repaired iceA1 of 60190 in pSYX22 |
This study |
| Primers |
|
|
| IceA60A-F |
CACACATATGTGTGGTGTGCGTGGCAACTC |
Forward |
| IceA60T-F |
TAGCCATATGGCTAAAGAATTTAATTTGGAG |
Forward |
| IceACH4-R |
GTTGGATCCGTCGACGTAGTTCATTGCAACCG |
Reverse |
| IceA-F |
GTTGGATCCTTAAGGAGGTTTAACATATGAGTAAAAGTAAAAAG |
Forward |
| IceA-R |
GTTGGATCCGTCGACGTAGTTCATTGCAACC |
Reverse |
| Fix-F |
GGTCTGCAGCTAGGTAATGGGGGGAGTTGGTGCAGG |
Forward |
| Fix-R |
TAGCTGCAGTCCTTGGTATTTTCC |
Reverse |
