Figure 5.

CBF-A mutant proteins and their G′2 3′-tail d(CGG)₇ destabilization activity. The conserved motifs marked in the scheme of wild-type CBF-A are: RNP2₁, MFVGGL; RNP1₁, SRGFGFIL; RNP2₂, IFVGGL; RNP1₂, RRGFVFIT; the putative ATP/GTP-binding domain, GSTNYGKS. The upper numbers in the wild-type protein scheme denote positions of residues at the boundaries of the conserved domains whereas the lower italicized numbers indicate the boundaries of segments deleted from each motif. Schemes show segments truncated or deleted in each mutant protein. The presented relative G′2 3′-tail d(CGG)₇ destabilization activity of each mutant protein is an average of the indicated number of independent determinations.