Table 1. Deletion of the RNP11 box or the ATP/GTP-binding motif does not affect binding or stabilization of G′2 5′-tail TeR2.
CBF-A protein | % G′2 5′-tail TeR2 binding (N) | %G′2 5′-tail TeR2 stabilization (N) |
---|---|---|
wt | 100 | 100 |
ΔR11 | 92.8 ± 3.1 (4) | 96.9 ± 24.4 (4) |
1–260 | 78.4 ± 1.1 (4) | 84.4 ± 11.0 (3) |
Wild-type (wt) or mutant CBF-A proteins were assayed for their ability to bind 5′-32P G′2 5′-tail TeR2 DNA as described in Materials and Methods. In parallel, the relative capacity of the proteins to stabilize 5′-32P G′2 5′-tail TeR2 DNA against denaturation for 7 min at 51°C was assayed as detailed in the legend to Figure 2B.