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. 2002 Sep 1;30(17):3672–3681. doi: 10.1093/nar/gkf506

Table 1. Deletion of the RNP11 box or the ATP/GTP-binding motif does not affect binding or stabilization of G′2 5′-tail TeR2.

CBF-A protein % G′2 5′-tail TeR2 binding (N) %G′2 5′-tail TeR2 stabilization (N)
wt 100 100
ΔR11 92.8 ± 3.1 (4) 96.9 ± 24.4 (4)
1–260 78.4 ± 1.1 (4) 84.4 ± 11.0 (3)

Wild-type (wt) or mutant CBF-A proteins were assayed for their ability to bind 5′-32P G′2 5′-tail TeR2 DNA as described in Materials and Methods. In parallel, the relative capacity of the proteins to stabilize 5′-32P G′2 5′-tail TeR2 DNA against denaturation for 7 min at 51°C was assayed as detailed in the legend to Figure 2B.