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Room temperature hybridizations of 5′ rhodamine-labeled LNAs. LNA probes (50 nM in 50 mM HEPES, pH 7.5, 100 mM NaCl) were mixed thoroughly with the respective amounts of DNA targets and the steady-level FP was recorded in a fluorometer. Shown are the titration curves for (A) LNA 601, 5′ Rho-GTCGCC; (B) LNA 602, 5′ Rho-GTCACC; (C) LNA 603, 5′ Rho-CATGCC; and (D) LNA 604, 5′ Rho-TATGCC with their perfect-match (filled squares) and single-base mismatch (empty squares) DNA targets.
