Table 1. Melting temperatures and FP dynamic ranges of 5′-Rho LNAs.
| LNA name, sequence (5′ to 3′) | Perfect-match Tm (°C) | FP dynamic range (mP) | Mismatch Tm (°C) and position |
|---|---|---|---|
| LNA601, Rho-GTCGCC | 53 | 110 | <0, G/T center |
| LNA602, Rho-GTCACC | 50 | 140 | <0, A/C center |
| LNA603, Rho-CATGCC | 26 | 35 | ND |
| LNA604, Rho-TATGCC | 21 | 60 | ND |
| LNA605, Rho-TCTGAC | 26 | 60 | ND |
| LNA606, Rho-GCGGAG | 62 | 110 | 31, G/T center; <0, G/A center |
| LNA607, Rho-ACGGAG | 53 | 110 | 24, G/T center; <0, G/A center |
| LNA608, Rho-ATGGTG | 43 | 115 | 17, G/T center; <0, G/A center |
| LNA609, Rho-AAGTAG | 25 | 90 | ND |
| LNA610, Rho-TAGTAT | 19 (300 nM) | 85 (300 nM) | ND |
| LNA701, Rho-CCCGCCC | >85 (250 nM) | 55 (250 nM) | 72, G/T center |
| LNA702, Rho-TATTTTA | 10 (300 nM) | 35 (300 nM) | ND |
| LNA709, Rho-TAAAATA | 15 | 85 | ND |
| LNA713, Rho-AAGTAGT | 34 | 130 | ND |
| LNA714, Rho-TAGTATT | 18 | 110 | ND |
| LNA715, Rho-TAGTATG | 32 | 120 | <0, T/G center |
| LNA807, Rho-TAGTATTT | 28 | 130 | <0, T/G center |
Unless otherwise indicated, probe and target concentrations were 50 nM; ND, not determined; <0, no LNA/DNA duplex was detected even at the lowest temperatures employed (4°C).