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. 1993 Oct;34(10):1348–1350. doi: 10.1136/gut.34.10.1348

Helicobacter pylori: comparison of DNA fingerprints provides evidence for intrafamilial infection.

K B Bamford 1, J Bickley 1, J S Collins 1, B T Johnston 1, S Potts 1, V Boston 1, R J Owen 1, J M Sloan 1
PMCID: PMC1374539  PMID: 8244100

Abstract

Although a high prevalence of antibodies to Helicobacter pylori has been documented within families, culture and DNA typing of strains from infected children and their parents has not been evaluated. This study aimed to analyse H pylori infection within family groups. Endoscopy, gastric biopsy, and H pylori culture were performed on all eight parents of four children who presented with dyspepsia and who had a positive H pylori culture. All biopsy specimens were cultured on Columbia based blood agar under microaerophilic conditions for four days. The DNA from each strain was extracted and electrophoretic patterns were compared after digestion with restriction endonucleases Hae III or Hind III. Ribotyping using a biotinylated cDNA probe prepared from 16S and 23S rRNA of H pylori NCTC 11638 was also used. Seven of the parents were positive for H pylori on urease testing, histology, and on culture. DNA typing showed the same or a similar strain to be present in at least two family members in three of the four family groups. In family 1, the mother, father, and child all had an identical strain; in family 2, father and son had a similar related strain; father and mother had the same strain in family 3; and all strains were unique in family 4. These data provide evidence for either intrafamilial cross infection or a common source of infection within family groups.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Drumm B., Perez-Perez G. I., Blaser M. J., Sherman P. M. Intrafamilial clustering of Helicobacter pylori infection. N Engl J Med. 1990 Feb 8;322(6):359–363. doi: 10.1056/NEJM199002083220603. [DOI] [PubMed] [Google Scholar]
  2. Forman D., Newell D. G., Fullerton F., Yarnell J. W., Stacey A. R., Wald N., Sitas F. Association between infection with Helicobacter pylori and risk of gastric cancer: evidence from a prospective investigation. BMJ. 1991 Jun 1;302(6788):1302–1305. doi: 10.1136/bmj.302.6788.1302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Jones D. M., Eldridge J., Fox A. J., Sethi P., Whorwell P. J. Antibody to the gastric campylobacter-like organism ("Campylobacter pyloridis")--clinical correlations and distribution in the normal population. J Med Microbiol. 1986 Aug;22(1):57–62. doi: 10.1099/00222615-22-1-57. [DOI] [PubMed] [Google Scholar]
  4. Lee A., Fox J. G., Otto G., Dick E. H., Krakowka S. Transmission of Helicobacter spp. A challenge to the dogma of faecal-oral spread. Epidemiol Infect. 1991 Aug;107(1):99–109. doi: 10.1017/s095026880004872x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Nomura A., Stemmermann G. N., Chyou P. H., Kato I., Perez-Perez G. I., Blaser M. J. Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii. N Engl J Med. 1991 Oct 17;325(16):1132–1136. doi: 10.1056/NEJM199110173251604. [DOI] [PubMed] [Google Scholar]
  6. Nwokolo C. U., Bickley J., Attard A. R., Owen R. J., Costas M., Fraser I. A. Evidence of clonal variants of Helicobacter pylori in three generations of a duodenal ulcer disease family. Gut. 1992 Oct;33(10):1323–1327. doi: 10.1136/gut.33.10.1323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Oderda G., Vaira D., Holton J., Ainley C., Altare F., Boero M., Smith A., Ansaldi N. Helicobacter pylori in children with peptic ulcer and their families. Dig Dis Sci. 1991 May;36(5):572–576. doi: 10.1007/BF01297021. [DOI] [PubMed] [Google Scholar]
  8. Oudbier J. H., Langenberg W., Rauws E. A., Bruin-Mosch C. Genotypical variation of Campylobacter pylori from gastric mucosa. J Clin Microbiol. 1990 Mar;28(3):559–565. doi: 10.1128/jcm.28.3.559-565.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Owen R. J., Bickley J., Lastovica A., Dunn J. P., Borman P., Hunton C. Ribosomal RNA gene patterns of Helicobacter pylori from surgical patients with healed and recurrent peptic ulcers. Epidemiol Infect. 1992 Feb;108(1):39–50. doi: 10.1017/s0950268800049487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Owen R. J., Hunton C., Bickley J., Moreno M., Linton D. Ribosomal RNA gene restriction patterns of Helicobacter pylori: analysis and appraisal of Hae III digests as a molecular typing system. Epidemiol Infect. 1992 Aug;109(1):35–47. [PMC free article] [PubMed] [Google Scholar]
  11. Parsonnet J., Friedman G. D., Vandersteen D. P., Chang Y., Vogelman J. H., Orentreich N., Sibley R. K. Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med. 1991 Oct 17;325(16):1127–1131. doi: 10.1056/NEJM199110173251603. [DOI] [PubMed] [Google Scholar]
  12. Prewett E. J., Bickley J., Owen R. J., Pounder R. E. DNA patterns of Helicobacter pylori isolated from gastric antrum, body, and duodenum. Gastroenterology. 1992 Mar;102(3):829–833. doi: 10.1016/0016-5085(92)90165-u. [DOI] [PubMed] [Google Scholar]
  13. Shames B., Krajden S., Fuksa M., Babida C., Penner J. L. Evidence for the occurrence of the same strain of Campylobacter pylori in the stomach and dental plaque. J Clin Microbiol. 1989 Dec;27(12):2849–2850. doi: 10.1128/jcm.27.12.2849-2850.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Simor A. E., Shames B., Drumm B., Sherman P., Low D. E., Penner J. L. Typing of Campylobacter pylori by bacterial DNA restriction endonuclease analysis and determination of plasmid profile. J Clin Microbiol. 1990 Jan;28(1):83–86. doi: 10.1128/jcm.28.1.83-86.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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