Fig 2.
Self-assembly products and digital image reconstructions of SbsB (see A and C for comparison) and fusion protein (S1)3S1B1 (B and D). (A and B) The crystalline sheets were formed in suspension and negatively stained with uranyl acetate for TEM. The arrows indicate the base vectors of the oblique p1 lattice. (Bars = 50 nm.) (C and D) The digital image reconstructions were made by Fourier processing of electron micrographs (not identical to the ones shown in A and B). The region of highest protein mass in the SbsB lattice is the SLH-domain (C, arrow). In the lattice of the fusion protein, streptavidin showed up as additional protein mass (D, thick arrow) attached to the SLH domain. (Bars = 10 nm.)