Fig 2.

Genetic interactions between RPA43 and RPA14 genes. (A) Positions and nature of the mutated residues in the rpa43 thermosensitive mutant are indicated. In A43-18, the C terminus of the protein is replaced by a short divergent amino acid sequence (thick line). Growth phenotype at different temperatures conferred by these different mutant alleles is summarized on the right. (B) Multicopy suppression. The rpa43-6, rpa43-18, and rpa43-4 mutant strains were transformed with a centromeric plasmid carrying the wild-type RPA43 gene (lanes 1, 4, and 7), with the empty pFL44 multicopy plasmid as a control (lanes 2, 5, and 8), or with the pFL44 vector harboring the RPA14 gene (lanes 3, 6, and 9). Growth of two dilutions of transformants was monitored at restrictive temperature.