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Western blotting analysis, showing the cleavage of YFP-Bid-CFP by caspase 8. Lane 1, control (no caspase 8) and lane 2, extract of COS 7 cells incubated with 1,000 units of caspase 8 for 22 h at 25°C. Extracts were separated by SDS/PAGE on a 10% polyacrylamide gel and subjected to Western blotting with an anti-GFP Ab. The upper arrow indicates YFP-Bid-CFP. The middle arrow indicates C-terminal Bid-CFP-fused protein, and the bottom arrow indicates YFP-N-terminal Bid-fused protein.
