Table 1.
The percentage of apoptotic cells that presented mitochondrial localization of cleaved Bid
| Protein
|
Apoptosis inducers | ||
|---|---|---|---|
| Fas | Tm | Aβ | |
| YFP-linker-CFP | 0 | 0 | 0 |
| YFP-Bid-CFP | 94 (±7.0) | 2 (±0.3) | 3 (±2.5) |
SK-N-SH cell lines expressing YFP-linker-CFP or YFP-Bid-CFP were stimulated by each of cell death inducers, anti-Fas antibody (Fas), Tm, or Aβ. The concentration of each inducer is described in Materials and Methods. The number of apoptotic cells was counted after stimulation. We observed >100 dead cells in each case and calculated standard deviation (performing this experiment more than three times).