Fig 5.
Effect of MDL-A on osteoclast formation in vitro. Osteoblast-like cells (1 × 104 per well) obtained from mouse calvaria and nucleated bone marrow cells (2 × 105 per well) were cocultured in a 48-well plate with 0.4 ml of α-MEM containing 10% FCS with 20 ng/ml rhIL-6 (containing 50 ng/ml rhIL-6R), 10 ng/ml rhIL-11, 100 ng/ml recombinant human LIF, 50 ng/ml rhIL-1, or 100 nM 1α,25(OH)2D3 in the presence (filled columns) or absence (open columns) of 30 μM MDL-A. Cultures were performed in duplicate over 4 days. Data are expressed as mean ± SEM (n = 3–4). Statistical significance: **, P < 0.01 vs. corresponding control.