Fig 2.

Concentration-dependent inhibition of hdm2-catalyzed p53 ubiquitination by compounds 1–3. A is a representative fluorescent image of p53-(Og-Ub)_n captured by a Storm 860 digital imager in the presence of compound 1 from 200–0.16 μM (as a 2.5-fold serial dilution). The rightmost lanes represent reactions run in the absence of inhibitor. The integrated intensities of the p53-(Og-Ub)_n bands in each lane were quantified, plotted, and fit to an inhibition isotherm to produce the concentration-response plot illustrated in B. The symbols in B represent the data for compounds 1 (•), 2 (○), and 3 (□).