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Chk1 is required for the IR-induced G2 checkpoint. Cells were mock-transfected (control) or transfected with double-strand siRNAs specific for either Chk1 or luciferase for 36 h. Cells were mock- or γ-irradiated, and 1 h later were harvested for Western blotting or were costained for DNA content and histone H3 phosphorylation and analyzed by flow cytometry (22). Percent mitotic cells is indicated.
