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Characterization of RLC-labeled muscle fibers. (A) RLC content determined from urea/polyacrylamide gels (2). (B) Ca sensitivity [1−T(pCa 9)/T(pCa 4.5)], where T is isometric tension. Native fibers were partially extracted (Ex 1), reconstituted with donor-labeled RLC (D), extracted to remove the remaining unlabeled RLC (Ex 2), and then reconstituted with acceptor-labeled RLC (DA).
