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Complex IV is the first to decrease in heme-deficient human brain cells. Heme deficiency was induced by incubation with 10 μM NMP. The cells were harvested and prepared for analysis at different intervals. The level of COXII (subunit II of complex IV), NOS, and APP were evaluated by SDS/PAGE followed by Western blotting. The data are from one of three independent experiments with U373 cells.
