Fig 7.

Heme deficiency compromises proliferation in human astrocytoma. Heme deficiency was induced as described in Fig. 6 in U373 cells. The cells were induced to proliferate by replacing the week-old medium with fresh complete medium. Heme-sufficient (a) and heme-deficient (c) cells seemed completely normal before the old medium was replaced by the fresh medium. Heme-sufficient cells were not affected by the fresh medium (b), whereas heme deficiency impaired cellular response to serum in heme-deficient cells (d) and the cells died within 7 h. Shown is one representative experiment of four.