Fig 5.
Deletion of mks1 and the addition of amino acids to growth media primarily affect the internal trafficking of Gap1p, not its endocytosis. (A) Wild type (CKY759), mks1Δ (CKY763), end3Δ (CKY765), and end3Δ mks1Δ (CKY766) were grown to exponential phase in minimal ammonia medium and assayed for Gap1p activity by [14C]citrulline uptake. (B) Wild type (CKY759) and end3Δ (CKY765) were grown to exponential phase and treated with 0.75 mM of the indicated amino acid or analog for 2 h before measurement of Gap1p activity by [14C]citrulline uptake. All strains contained PADH1-GAP1-HA.