Table 2.

Glutamate, glutamine, glutamate + glutamine, and total amino acid content of gdh1Δ, gln1-9142, and mks1Δ

Strain	Amino acid content (nmol/OD600)
	Glu	Gln	Glu + Gln	Total amino acids
Wild type	19	23	42	96
gdh1Δ	17	13	30	59
gln1-9142	24	10	34	96
mks1Δ	43	28	71	138
Nitrogen-free (4 h)	13	1	14	26

Wild type (CKY443), gdh1Δ (CKY757), gln1-9142 (CKY767), and mks1Δ (CKY758) were grown to exponential phase on minimal ammonia media at 24°C. For the nitrogen-free sample, wild-type cells were shifted from ammonia medium to nitrogen-free medium for 4 h before harvesting. Sample preparation and analysis were performed as described in Materials and Methods. One OD₆₀₀ unit equals 2 × 10⁷ cells.