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TaqMan RT-PCR results for biglycan and embryonic myosin (MYH3). For each gene, profiles of product appearance from two DMD (red traces) and two unaffected biopsies (black traces) are shown. The x axis indicates the number of cycles to product appearance. In both cases, product amplification from the DMD biopsy preceded amplification from the unaffected biopsy, verifying that both biglycan and embryonic myosin are overexpressed in DMD muscle versus unaffected muscle.
