Fig 4.

Effector TCR^Tg CD4⁺ T cells do not expel irrelevant MHC class II/peptide complexes from the center of the contact sites with DCs. In vitro activated TCR^Tg CD4⁺ T cells (T) were mixed with A^bIi⁻ DCs (DC) expressing agonist A^bPCCVA-YFP and null A^bEp-CFP complexes. (A–D) A single 2D optical section in the center of the cell conjugate. (E–G) A 3D view of the cell–cell contact. (A) DIC image. (B and E) A^bPCCVA-YFP only (green). (C and F) A^bEp-CFP only (blue). (D and G) A^bPCCVA-YFP and A^bEp-CFP overlay. This image is representative for 39 contacts analyzed in three independent experiments (Table 1).