Table 1.
Oligonucleotide primers (first and second, nested set) for PCR amplification of cDNAs obtained after reverse transcription from HFFF2 cells, paraffin-embedded human testicular biopsies, and cells of the wall of seminiferous tubules collected after LCM
| Target
|
|
Primers | bp
|
|
|---|---|---|---|---|
| Sense | Antisense | |||
| COX2 | 1 | 5′-GCAAATCCTTGCTGTTCC-3′ | 5′-GGAGGAAGGGCTCTAGTA-3′ | 368 |
| 2 | 5′-CCATGTCAAAACCGTGG-3′ | 5′-TAGGAGAGGTTGGAGAA-3′ | 326 | |
| PAR2 | 1 | 5′-CATCCTGCTAGCAGCCTC-3′ | 5′-ACCTCTGCACACTGAGGC-3′ | 480 |
| 2 | 5′-GATGGCACATCCCACGTC-3′ | 5′-GGCATGTATGTGATAGGC-3′ | 288 | |
| PPARγ | 1 | 5′-TCTGGCCCACCAACTTTG-3′ | 5′-ACAAGCATGAACTCCATA-3′ | 356 |
| 2 | 5′-GATTACAAGTATGACCTG-3′ | 5′-GCTTTATCTCCACAGAC-3′ | 164 | |