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Inverse PCR scheme. Locations of PCR primers and restriction sites around intron 13 of the E2A gene. G, BglII; X, XbaI; B, BamH1; A, location of invA and invA-1 primers; B, location of invB and invB-1 primers. InvB-2, 3, 4, and 5 are located just to the right of invB-1. C corresponds to invC and invC-1; D corresponds to invD and invD-1, respectively. InvC-2, 3, 4, and 5 are the complement of invB-5, 4, 3, and 2, respectively.
