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. 2002 Nov 1;99(23):15101–15106. doi: 10.1073/pnas.222481199

Fig 5.

Fig 5.

Guthrie card analysis of patient #295. The second-round PCR products are shown for the dilution series of diagnostic patient DNA (lanes 1–4, 1:10 dilution series, starting at 50 ng/μl patient DNA in lane 1). Guthrie card segments for control (C) cards are shown with segments from patient #295 (P) and a no-DNA blank (B). A single-lane positive shown was sequenced and determined to match the sequence from the patient. Results were similar for patient #58.