Fig 2.

Expression of endogenous ADAR1 and ADAR2 during HDV replication in Huh7 cells. (A) Western analyses were performed on total cell lysates expressing replicating-HDV RNA (HDV+) or control vector (HDV−) 4–10 days posttransfection and immunoblotted with either anti-ADAR1 (Upper) or anti-ADAR2 (Lower) polyclonal antisera. std, standard. (B) Efficient editing was occurring during the course of replication. (Upper) A Western analysis performed with anti-delta antigen polyclonal antiserum showing the edited (HDAg-L) and unedited (HDAg-S) products. (Lower) A Northern blot probed to detect the HDV genome.