Fig 3.

Overexpression of HA-tagged ADAR1-L and ADAR1-S during HDV replication. Western analyses were performed on total cell lysates expressing replicating-HDV RNA and either HA-tagged ADAR1-L or ADAR1-S. Posttransfection (3, 5, and 7 days), the samples were blotted with either anti-HA monoclonal antibody (Upper) to monitor protein expression or anti-delta antigen polyclonal antiserum (Lower) to monitor editing. The relative expression of the HA-tagged ADARs was obtained by comparison with a HA-tagged ADAR1 standard. An equal aliquot of this standard was loaded on all of the anti-HA Westerns, and the signal obtained for this standard was given a value of 10 (27).