Fig 5.

siRNAs targeted against ADAR1 decreased ADAR1 endogenous expression and activity. (Top) An anti-ADAR1 Western analysis to detect endogenous ADAR1 expression when siRNAs targeted against either ADAR2 or both forms of ADAR1 (siADAR1-L+S) or ADAR1-L alone (siADAR1-L) were introduced into HEK293 cells. Each siRNA was tested in duplicate. (Middle) An anti-delta antigen (HDAg) Western analysis to monitor the corresponding editing activity assayed with an HDV amber/W reporter, pSS140 (28). (Bottom) An anti-β-actin Western analysis to serve as a total protein loading control. ADAR expression was normalized to β-actin expression.