Fig 1.
Schematic representation of the HSV-1 (F) genome and gene manipulations in glycoprotein C (gC), glycoprotein B (gB), and glycoprotein D (gD). Line 1, sequence arrangement of HSV-1 genome. The rectangular boxes represent the inverted repeat sequences ab and b′a′ flanking the unique long (UL) sequence and inverted repeat c′a′ and ca flanking the unique short (US) sequence. Line 2, sequence arrangement of the domains of the glycoprotein C, signal peptide (SP) domain, and heparan sulfate (HS)-binding domain of gC are highlighted. Line 3, human IL-13 with signal peptide that replaced the N-terminal segment of 148 aa of gC. Line 4, sequence arrangement of the polylysine domain of gB. Line 5, schematic representation of recombinant HSV-1(F) genome, in which the N-terminal domain of gC was replaced with IL-13, and the polylysine domain (from codon 68 to codon 77) of gB was deleted. Line 6, sequence arrangement of glycoprotein J (gJ), glycoprotein D (gD), and glycoprotein I (gI) in US. Line 7, replacement of gD with the immediately early promoter of cytomegalovirus to enable the expression of gI. Line 8, schematic representation of recombinant HSV-1(F) genome, in which the N-terminal domain of gC was replaced with IL-13, the polylysine domain of gB was deleted, and IL-13 was inserted after amino acid 24 of gD. Line 9, a polylinker XhoI-BglII-EcoRI-KpnI was inserted after amino acid 24 of gD, and then IL-13 was inserted into the XhoI and KpnI sites of gD.